Pdf evaluation of a dengue ns1 capture elisa assay for. Transmission is by mosquito, principally aedes aegypti and aedes albopictus. Recently, two commercial elisas that are based on these different strategies have been released. We compared two generations of panbio brisbane, australia commercial kits for ns1 antigen capture for early diagnosis of dengue. The performances of the mrl dengue fever virus immunoglobulin m igm capture enzymelinked immunosorbent assay elisa and. Panbio dengue igm capture elisa, is a test for the qualitative detection of igm antibodies to dengue antigen, as an aid in the clinical laboratory diagnostics of patients with clinical symptomps consistent with dengue fewer. Comparison of panbio dengue duo enzymelinked immunosorbent. This dengue virus igm capture elisa has excellent specificity and sensitivity for the quantitative determination of igm antibodies against dengue virus.
Dengue igm levels obtained by 2 different testing kits in 12 samples from 10 confirmed dengue imported cases in new caledonia, 20052006. Dengue virus igm capture elisa the native antigen company. A commercially available capture enzymelinked immunosorbent assay elisa for the detection of specific immunoglobulin m igm and igg antibodies produced during dengue infection panbio dengue. Evaluation of commercially available antidengue virus. Its laboratory diagnosis, with special emphasis on. Panbio dengue igm capture elisa rapid point of care diagnostics. The most widely used method for diagnosing dengue is an enzymelinked immunosorbent assay elisa which measures antidenv igm or igg antibodies in patient serum. Relevant identified uses of the substance or mixture and uses advised against not available not available safety data sheet sds according to regulation ec no 4532010 panbio dengue igm capture elisa doc.
Comparison of two commercial elisa kits for the detection. Pdf comparison of panbio dengue duo igm and igg capture. Eden01m eden01m05 intended use the panbio dengue igm capture elisa is for the qualitative detection of igm antibodies to dengue antigen in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with dengue fever. This assay is specific for igm and uses a flavivirus group monoclonal horseradish peroxidase conjugate with tmb as substrate. The panbio dengue igm capture elisa is for the qualitative detection of igm antibodies to dengue antigen in serum, as an aid in the clinical laboratory. Igm capture elisa for detection of igm antibodies to dengue virus. Pdf comparison of panbio dengue igm elisa assay with. The test improvement resulted in a highly sensitive and specific test suitable for use as a firstline test in the field.
Not for sale or distribution in the united states of. The venture technologies dengue igm and igg dot blot assays and the panbio dengue duo igm and igg capture elisa were compared. The detection rate by panbio dengue ns1 antigen capture elisa in the absence of igm was 91. The panbio dengue igm capture elisa should be used in conjunction with other dengue serology. Comparison of igm capture elisa with a commercial rapid. Panbio dengue igm capture elisawash buffer, sample diluent, antigen diluent buffer, dengue 14 antigen, hrp conjugate 1. Dengue, igm detection, niv igm capture elisa, panbio rapid immunochromatographic card test. Enzyme immunoassay for the detection of igm antibody to dengue virus. These antibodies are not reliably detectable until 34 days post symptom onset pso 12 and requires the collection of a second blood sample 1421 days after the first visit for. Evaluation of dengue ns1 antigen rapid tests and elisa.
The venture technologies dengue igm and igg dot blot assays and the. Comparison of two generations of the panbio dengue. Sd bioline dengue igm capture elisa rapid point of care. Assays for the serological diagnosis of dengue infections dengue igmcapture elisa panbio dengue iggindirect elisa panbio dengue ns1 antigen detection elisa panbio trainer. A number of commercial elisa for dengue diagnosis have recently become available, though direct comparison between these assays have not been published.
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